{"id":1524,"date":"2020-08-14T12:55:59","date_gmt":"2020-08-14T15:55:59","guid":{"rendered":"https:\/\/edctecnologia.com.br\/reduced-susceptibility-mechanism-to-cefiderocol-a-siderophorecephalosporin-among-clinical-isolates-from-global-surveillance-program-sidero-wt-2014\/"},"modified":"2020-08-28T01:04:21","modified_gmt":"2020-08-28T04:04:21","slug":"reduced-susceptibility-mechanism-to-cefiderocol-a-siderophorecephalosporin-among-clinical-isolates-from-global-surveillance-program-sidero-wt-2014","status":"publish","type":"post","link":"https:\/\/edctecnologia.com.br\/es\/reduced-susceptibility-mechanism-to-cefiderocol-a-siderophorecephalosporin-among-clinical-isolates-from-global-surveillance-program-sidero-wt-2014\/","title":{"rendered":"Reduced susceptibility mechanism to cefiderocol among clinical isolates from global surveillance program (SIDERO-WT-2014)"},"content":{"rendered":"<p><strong>Objectives<\/strong><\/p>\n<p>To investigate possible mechanistic factors to explain cefiderocol (CFDC) non-susceptibility, we characterized 38 clinical isolates with cefiderocol MIC of &gt;4 \u03bcg\/mL from a multi-national surveillance study.<\/p>\n<p><strong>Methods<\/strong><\/p>\n<p>The minimum inhibitory concentration (MIC) measurement in the presence of \u03b2-lactamase inhibitors and whole genome sequencing were performed.<\/p>\n<p><strong>Results<\/strong><\/p>\n<p>The MIC decrease of CFDC by \u03b2-lactamase inhibitors were observed against all of the test isolates. Among 38 isolates, NDM or PER genes were observed in 5 and 25 isolates, respectively. No other \u03b2-lactamases responsible for high MIC were identified in other 8 isolates. MIC of CDFC against Escherichia coli isogenic strains introduced with NDM and PER \u03b2-lactamase increased by \u226516-fold, suggesting the contribution of NDM and PER to the non-susceptibility to CFDC. Against NDM producers, \u22658-fold MIC increase was observed only when both serine- and metallo-type beta-lactamase inhibitors were added. In addition, many of PER- or NDM-producers remained susceptible to CFDC. These results suggested that the presence of only NDM or PER would not lead to non-susceptibility to CFDC and that multiple factors would be related with CFDC resistance.<\/p>\n<p><strong>Conclusions<\/strong><\/p>\n<p>Multiple factors including NDM and PER could be related to reduced susceptibility to CFDC.<\/p>\n<p>&nbsp;<\/p>\n<p>Para ter acesso ao artigo completo, baixe <strong><a href=\"https:\/\/edctecnologia.com.br\/wp-content\/uploads\/2020\/08\/1-s2.0-S2213716520301843-main.pdf\">aqui<\/a>.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Objectives To investigate possible mechanistic factors to explain cefiderocol (CFDC) non-susceptibility, we characterized 38 clinical isolates with cefiderocol MIC of &gt;4 \u03bcg\/mL from a multi-national surveillance study. Methods The minimum inhibitory concentration (MIC) measurement in the presence of \u03b2-lactamase inhibitors and whole genome sequencing were performed. Results The MIC decrease of CFDC by \u03b2-lactamase inhibitors [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":746,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[33],"tags":[],"solucao":[60],"tipo_post":[52],"tipo_evento":[],"class_list":["post-1524","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-edc-es","solucao-biovia-pipeline-pilot-es","tipo_post-papers-es-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/posts\/1524","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/comments?post=1524"}],"version-history":[{"count":1,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/posts\/1524\/revisions"}],"predecessor-version":[{"id":1530,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/posts\/1524\/revisions\/1530"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/media\/746"}],"wp:attachment":[{"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/media?parent=1524"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/categories?post=1524"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/tags?post=1524"},{"taxonomy":"solucao","embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/solucao?post=1524"},{"taxonomy":"tipo_post","embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/tipo_post?post=1524"},{"taxonomy":"tipo_evento","embeddable":true,"href":"https:\/\/edctecnologia.com.br\/es\/wp-json\/wp\/v2\/tipo_evento?post=1524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}