{"id":1524,"date":"2020-08-14T12:55:59","date_gmt":"2020-08-14T15:55:59","guid":{"rendered":"https:\/\/edctecnologia.com.br\/reduced-susceptibility-mechanism-to-cefiderocol-a-siderophorecephalosporin-among-clinical-isolates-from-global-surveillance-program-sidero-wt-2014\/"},"modified":"2020-08-28T01:04:21","modified_gmt":"2020-08-28T04:04:21","slug":"reduced-susceptibility-mechanism-to-cefiderocol-a-siderophorecephalosporin-among-clinical-isolates-from-global-surveillance-program-sidero-wt-2014","status":"publish","type":"post","link":"https:\/\/edctecnologia.com.br\/es\/reduced-susceptibility-mechanism-to-cefiderocol-a-siderophorecephalosporin-among-clinical-isolates-from-global-surveillance-program-sidero-wt-2014\/","title":{"rendered":"Reduced susceptibility mechanism to cefiderocol among clinical isolates from global surveillance program (SIDERO-WT-2014)"},"content":{"rendered":"
Objectives<\/strong><\/p>\n To investigate possible mechanistic factors to explain cefiderocol (CFDC) non-susceptibility, we characterized 38 clinical isolates with cefiderocol MIC of >4 \u03bcg\/mL from a multi-national surveillance study.<\/p>\n Methods<\/strong><\/p>\n The minimum inhibitory concentration (MIC) measurement in the presence of \u03b2-lactamase inhibitors and whole genome sequencing were performed.<\/p>\n Results<\/strong><\/p>\n The MIC decrease of CFDC by \u03b2-lactamase inhibitors were observed against all of the test isolates. Among 38 isolates, NDM or PER genes were observed in 5 and 25 isolates, respectively. No other \u03b2-lactamases responsible for high MIC were identified in other 8 isolates. MIC of CDFC against Escherichia coli isogenic strains introduced with NDM and PER \u03b2-lactamase increased by \u226516-fold, suggesting the contribution of NDM and PER to the non-susceptibility to CFDC. Against NDM producers, \u22658-fold MIC increase was observed only when both serine- and metallo-type beta-lactamase inhibitors were added. In addition, many of PER- or NDM-producers remained susceptible to CFDC. These results suggested that the presence of only NDM or PER would not lead to non-susceptibility to CFDC and that multiple factors would be related with CFDC resistance.<\/p>\n